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The In Vivo Imaging Page provides a Discussion Forum and Links to information about the discipline of "In Vivo" Molecular Imaging with an emphasis on Pre-clinical Animal Models, not excluding Translational Imaging. Techniques covered are: Optical Imaging, Molecular Imaging, Fluorescence Imaging, Bioluminescence Imaging, MRI, PET, SPECT, CT, Ultrasound and Intravital Microscopy.
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IN VIVO IMAGING - WHITE PAPERS
How to use a Lentivirus as a vehicle for reporter gene transfection of cells.
Lentivirus protocol to make your own luciferase cell lines, Fast and easy.
Labeling cells with a genetically encoded reporter gene (firefly luciferase, red fluorescent protein, transferrin, thymidine kinase, etc.) is the number one choice for in vivo molecular imaging. Various transfection protocols to introduce foreign DNA in cells exist. A virus is nature’s own shuttle of genetic material and therefore viral infection is by far the most efficient transduction method even for resilient cell lines. A lentivirus Read more
In Vivo Optical Imaging Reporters
There is a plethora of choice in reporter tags for non-invasive in vivo optical imaging (fluorescence and bioluminescence) that are currently available from various commercial suppliers. This generates tremendous flexibility on one hand, but dilemma of choice on the other. This manuscript reviews the determining factors and will facilitate decision making using an interactive flow chart. Read more
D-Luciferin: in vivo imaging methods
D-luciferin [(S)-2-(6′-hydroxy-2′-benzothiazolyl)thiazoline-4-carboxylic acid] is the substrate of the North American firefly Photinus Pyralis luciferase and Clickbeetle Red and Clickbeetle Green luciferase. Luciferin is a low molecular weight (318.41 g/mole) organic compound that consists of a benzothiazole moiety attached to a thiazole carboxylic acid moiety. Luciferin is a small molecule which freely diffuses across membranes. When luciferin is injected in vivo Read more
Cell Culture Techniques for In Vivo Grafting
Mouse models for in vivo imaging of cancer established by in vivo grafting of syngeneic or xenogeneic cells in recipient animals are commonly practiced. Success of these studies relies upon solid cell culture technique. This article covers sterile technique, thawing and freezing, subculture and preparation of cells for grafting. Read moreMicrobubbles for in vivo ultrasound mouse imaging are encapsulated, inert gas filled colloidal particles. Upon intravenous injection, they are used as ultrasound contrast agents. Read more
Lab Animal Anesthesia - inhalation and injectable protocols
Inhalation anesthesia with isoflurane is quickly becoming the standard method of general anesthesia for rats and mice used in biomedical research and is imperative for in vivo imaging. Read more
IN VIVO IMAGING - IN THE NEWS
CEA-Leti’s New Imaging System for fDOT Ready for Commercialization, Use in Measuring and Treating Cancer
St. Jude Medical Completes Acquisition of LightLab Imaging
SonoSite Completes Acquisition of VisualSonics
Noninvasive MR Imaging of Blood Vessel Growth in Tumors Using Nanosized Contrast Agents
Turning Back the Cellular Clock: Method Developed for Tracking Adult Stem Cells as They Regress
Nanoparticles as Destructive Beacons to Zap Tumors
How Do Cells Die? Biophotonic Tools Reveal Real-Time Dynamics in Living Color
Replacing Hydrogen in Fluorescent Dyes Improves Detection Ability, Stability and Shelf Life
High-Speed Study of Zebrafish Larvae: New Technique Can Analyze Larvae in Seconds
Scientists Create Army of Tumor-Fighting Immune Cells and Watch as They Attack Cancer
Mice Cages Can Alter Rodents’ Brains and Skew Research Results, Study Finds
AVEO Pharmaceuticals’ Human Response Platform™ and Proprietary Mouse Models Featured in Nature Reviews Cancer
To Predict Atherosclerosis, Follow the Disturbed Blood Flow
Nanoparticles Shrink Tumors in Mice
Smaller tags cure the GFP blues
Researchers reduce risk of teratomas from stem cell transplants
More News...
MICAD DATABASE - NEW AGENTS
PET Agents
64Cu-PEI Target: Heparin sulfate proteoglycans
[11C]Methoxy-Sch225336 Target: Cannabinoid CB2 receptor (CB2R)
[11C]SU11274 Target: Mesenchymal-epithelial transition factor (MET)
[11C]SL25.1188 Target: Monoamine oxidase B (MAO-B)
SPECT Agents
67Ga-DOTA-GlyGlu-CycMSH Target: Melanocortin-1 receptor (MC1R)
[186Re]MAMA-HBP Target: Farnesyl disphosphate (pyrophosphate) synthase
[186Re]MAMA-BP Target: Farnesyl disphosphate (pyrophosphate) synthase
[125I]IodoDPA-713 Target: Peripheral-type benzodiazepine receptor (PBR), also known as translocator protein (TSPO)
[125I]-BH-Exendin(9-39) Target: Glucagon-like peptide-1 (GLP-1) receptor
125I-EGFR-Fab Target: Epidermal growth factor receptor (EGFR)
111In-[DTPA1-Lys3,Tyr4]-BN Target: Gastrin-releasing peptide receptor (GRPR)
111In-DOTA-E3-uroguanylin Target: Receptor guanylate cyclase C (GC-C)
99mTc-PR81 Target: Mucin
99mTc-DTPA-TOR Target: Estrogen receptor
Optical Agents
tAB50-Cy5 Target: Caspase-3, caspase-7
Legumain probe 1 Target: Legumain/Asparaginyl endopeptidase
AnxB12 Cys101,Cys260-IANBD Target: Phosphatidylserine
RECENT IN VIVO IMAGING PUBLICATIONS
- Evaluation of the radiation dose in micro-CT with optimization of the scan protocol.
- Validation of simple and robust protocols for high-resolution lung proton MRI in mice.
- Source intensity profile in noncontact optical tomography.
- Efficient Gene Transfer Into the Mouse Lung by Fetal Intratracheal Injection of rAAV2/6.2.
- Early detection of radiation therapy response in non-Hodgkin's lymphoma xenografts by in vivo (1)H magnetic resonance spectroscopy and imaging.
PubMed: CT in Vivo Imaging
- Preclinical and clinical evidence that 18FDG-PET/CT is a reliable tool for the detection of early molecular responses to erlotinib in head and neck cancer.
- Small-Animal PET/CT for Monitoring the Development and Response to Chemotherapy of Thymic Lymphoma in Trp53-/- Mice.
- TNF-alpha mRNA Expression Correlates with TGF-beta mRNA Expression In Vivo.
- 5-HT1A and 5-HT1B receptors differentially modulate rate and timing of auditory responses in the mouse inferior colliculus.
- Genetic Sphingosine Kinase 1 Deficiency Significantly Decreases Synovial Inflammation and Joint Erosions in Murine TNF-{alpha}-Induced Arthritis.
PubMed: MRI In Vivo Imaging
- Early detection of radiation therapy response in non-Hodgkin's lymphoma xenografts by in vivo (1)H magnetic resonance spectroscopy and imaging.
- A comparative study of age-related hearing loss in wild type and insulin-like growth factor I deficient mice.
- In vivo angiogenesis imaging of solid tumors by {alpha}v{beta}3-targeted, dual-modality micellar nanoprobes.
- Mapping of CBV changes in 5-HT(1A) terminal fields by functional MRI in the mouse brain.
- An MRI-based atlas and database of the developing mouse brain.
PubMed: Ultrasound In Vivo Imaging
- SSTR2-based Reporters for Assessing Gene Transfer into Non-Small Cell Lung Cancer: Evaluation using an Intrathoracic Mouse Model.
- Enhancement of antitumor vaccine in ablated hepatocellular carcinoma by high-intensity focused ultrasound.
- A microcomposite hydrogel for repeated on-demand ultrasound-triggered drug delivery.
- In vivo photoacoustic imaging of transverse blood flow by using Doppler broadening of bandwidth.
- A high-frequency, high frame rate duplex ultrasound linear array imaging system for small animal imaging.
PubMed: PET In Vivo Imaging
- PET imaging AND vivo AND mouse; +57 new citations
- 18[F]FDG small animal PET study of sorafenib efficacy in lymphoma preclinical models.
- Fluorine-18 labeled galactosylated chitosan for asialoglycoprotein-receptor-mediated hepatocyte imaging.
- A Dual Radiologic Contrast Agent Protocol for (18)F-FDG and (18)F-FLT PET/CT Imaging of Mice Bearing Abdominal Tumors.
- Evaluation of Two Internalizing Carcinoembryonic Antigen Reporter Genes for Molecular Imaging.
PubMed: SPECT In Vivo Imaging
- SSTR2-based Reporters for Assessing Gene Transfer into Non-Small Cell Lung Cancer: Evaluation using an Intrathoracic Mouse Model.
- SPECT AND vivo AND mouse; +70 new citations
- In vivo chemistry for pretargeted tumor imaging in live mice.
- PET imaging of fatty acid amide hydrolase in the brain: synthesis and biological evaluation of an (11)C-labelled URB597 analogue.
- Pre-clinical evaluation of a 3-nitro-1,2,4-triazole analogue of [(18)F]FMISO as hypoxia-selective tracer for PET.
PubMed: Intravital Microscopy In Vivo Imaging
- TRAF5 Deficiency Accelerates Atherogenesis in Mice by Increasing Inflammatory Cell Recruitment and Foam Cell Formation.
- Imaging of protein translocation in situ in skeletal muscle of living mice.
- Development of a liposomal delivery system for temperature-triggered release of a tumor targeting agent, Ln(III)-DOTA-phenylboronate.
- Triggered content release from optimized stealth thermosensitive liposomes using mild hyperthermia.
- Near-Infrared Fluorescent Probe for Imaging of Pancreatic beta Cells.
