Skip to Content

Welcome to:

The In Vivo Imaging Community







home
Image Map

â–ºClick on spheres for additional info

The In Vivo Imaging Page provides a Discussion Forum and Links to information about the discipline of "In Vivo" Molecular Imaging with an emphasis on Pre-clinical Animal Models, not excluding Translational Imaging. Techniques covered are: Optical Imaging, Molecular Imaging, Fluorescence Imaging, Bioluminescence Imaging, MRI, PET, SPECT, CT, Ultrasound and Intravital Microscopy.

Sign Up to access full Resources.

 


 

 


 

IN VIVO IMAGING - WHITE PAPERS

 

How to use a Lentivirus as a vehicle for reporter gene transfection of cells.

Lentivirus protocol to make your own luciferase cell lines, Fast and easy.

Labeling cells with a genetically encoded reporter gene (firefly luciferase, red fluorescent protein, transferrin, thymidine kinase, etc.) is the number one choice for in vivo molecular imaging. Various transfection protocols to introduce foreign DNA in cells exist. A virus is nature’s own shuttle of genetic material and therefore viral infection is by far the most efficient transduction method even for resilient cell lines. A lentivirus Read more

In Vivo Optical Imaging Reporters

There is a plethora of choice in reporter tags for non-invasive in vivo optical imaging (fluorescence and bioluminescence) that are currently available from various commercial suppliers. This generates tremendous flexibility on one hand, but dilemma of choice on the other. This manuscript reviews the determining factors and will facilitate decision making using an interactive flow chart. Read more

D-Luciferin: in vivo imaging methods

D-luciferin [(S)-2-(6′-hydroxy-2′-benzothiazolyl)thiazoline-4-carboxylic acid] is the substrate of the North American firefly Photinus Pyralis luciferase and Clickbeetle Red and Clickbeetle Green luciferase. Luciferin is a low molecular weight (318.41 g/mole) organic compound that consists of a benzothiazole moiety attached to a thiazole carboxylic acid moiety. Luciferin is a small molecule which freely diffuses across membranes. When luciferin is injected in vivo Read more

Cell Culture Techniques for In Vivo Grafting

Mouse models for in vivo imaging of cancer established by in vivo grafting of syngeneic or xenogeneic cells in recipient animals are commonly practiced. Success of these studies relies upon solid cell culture technique. This article covers sterile technique, thawing and freezing, subculture and preparation of cells for grafting. Read more

Microbubbles for in vivo ultrasound mouse imaging are encapsulated, inert gas filled colloidal particles. Upon intravenous injection, they are used as ultrasound contrast agents. Read more

Lab Animal Anesthesia - inhalation and injectable protocols

Inhalation anesthesia with isoflurane is quickly becoming the standard method of general anesthesia for rats and mice used in biomedical research and is imperative for in vivo imaging. Read more

MICAD DATABASE - NEW AGENTS

PET Agents

64Cu-PEI Target: Heparin sulfate proteoglycans

[11C]Methoxy-Sch225336 Target: Cannabinoid CB2 receptor (CB2R)

[11C]SU11274 Target: Mesenchymal-epithelial transition factor (MET)

[11C]SL25.1188 Target: Monoamine oxidase B (MAO-B)

 

SPECT Agents

67Ga-DOTA-GlyGlu-CycMSH Target: Melanocortin-1 receptor (MC1R)

[186Re]MAMA-HBP Target: Farnesyl disphosphate (pyrophosphate) synthase

[186Re]MAMA-BP Target: Farnesyl disphosphate (pyrophosphate) synthase

[125I]IodoDPA-713 Target: Peripheral-type benzodiazepine receptor (PBR), also known as translocator protein (TSPO)

[125I]-BH-Exendin(9-39) Target: Glucagon-like peptide-1 (GLP-1) receptor

125I-EGFR-Fab Target: Epidermal growth factor receptor (EGFR)

111In-[DTPA1-Lys3,Tyr4]-BN Target: Gastrin-releasing peptide receptor (GRPR)

111In-DOTA-E3-uroguanylin Target: Receptor guanylate cyclase C (GC-C)

99mTc-PR81 Target: Mucin

99mTc-DTPA-TOR Target: Estrogen receptor

 

Optical Agents

 tAB50-Cy5 Target: Caspase-3, caspase-7

Legumain probe 1 Target: Legumain/Asparaginyl endopeptidase

AnxB12 Cys101,Cys260-IANBD Target: Phosphatidylserine